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Tested Applications
| Positive Functional Assay detected in | human PBMCs |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| FUNCTIONAL ASSAY | FUNCTIONAL ASSAY : Assay Dependent |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
BK00002 targets CD3 in FC, Functional Assay applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
unknow 相同性解析による交差性が予測される生物種 |
| Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
| Calculated molecular weight | 207 aa, 23 kDa |
| GenBank accession number | BC049847 |
| Gene Symbol | CD3 |
| Gene ID (NCBI) | 916 |
| ENSEMBL Gene ID | ENSG00000198851 |
| Conjugate | Unconjugated |
| 来源 | Purified from CHO cell supernatant in an animal-free facility |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Purity | ≥98% (HPLC), ≥98% (SDS-PAGE) |
| Sterility | 0.22 µm filtration |
| UNIPROT ID | P07766 |
| Storage Buffer | Sterile PBS{{ptg:BufferTemp}}7.4 |
| Storage Conditions | For long-term storage, store at -80°C. Avoid repeated freeze-thaw cycles. For short-term use, store at 2-8°C for up to 2 weeks. |
| Endotoxin | <0.005 EU/mg |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD3 antibody BK00002 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |