Atlantic Blue™ Anti-Rat CD8a (OX-8)

CD8a Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

OX-8

Cat no : AB-65180

Synonyms

Cd8a, CD8a molecule



Tested Applications

Positive FC detected inWistar rat splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65180 targets CD8a in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen High-molecular-weight rat thymocyte glycoproteins
Full Name CD8a molecule
GenBank Accession NumberBC088126
Gene Symbol Cd8a
Gene ID (NCBI) 24930
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths404 nm / 458 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for Atlantic Blue™ CD8a antibody AB-65180Download protocol
Standard Protocols
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