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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human Human IgG1 Rabbit Recombinant Antibody (98804-3-RR)

    IHC staining of human tonsillitis using 98804-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98721-9-RR (Human IgG1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IgG1 Rabbit Recombinant Antibody (98804-3-RR)

    FC experiment of human PBMCs using 98804-3-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human Human IgG1 Rabbit RecAb (98804-3-RR, Clone: 251296E10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). The cells originate from CD11b-negative cells. Cells were incubated with FC Receptor Block prior to staining. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Anti-Human IgG1 Rabbit Recombinant Antibody

IgG1 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98804-3-RR
Clone No.251296E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

Human IgG1, IGHG1, Immunoglobulin heavy constant gamma 1

Formulation:  PBS, Azide
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98804-3-RR targets IgG1 in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6672

Product name: recombinant human IgG1-IgK protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10009498 of

Sequence: /

相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Gene Symbol IGHG1
Gene ID (NCBI) 3500
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Human Immunoglobulin G subclass 1 (IgG1) is the most abundant antibody subclass in human serum, constituting approximately 60-70% of total IgG. It serves as a critical mediator of the adaptive immune system's defense against pathogens. Human IgG1 represents a perfect balance of specificity, stability, and powerful immune-activating capacity. Its dominance in the biotherapeutic landscape is a testament to its versatility and effectiveness. This antibody does not recognize IgA2.

Protocols

Product Specific Protocols
FC protocol for IgG1 antibody 98804-3-RRDownload protocol
IHC protocol for IgG1 antibody 98804-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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