Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Transfected CHO cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98647-1-RR targets IFN-alpha 4/IFNA4 in FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4962 Product name: Recombinant Human IFNA4 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 24-189 aa of BC113642 Sequence: CDLPQTHSLGNRRALILLAQMGRISHFSCLKDRHDFGFPEEEFDGHQFQKAQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNDLEACVIQEVGVEETPLMNEDSILAVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD 相同性解析による交差性が予測される生物種 |
| Full Name | interferon, alpha 4 |
| GenBank accession number | BC113642 |
| Gene Symbol | IFNA4 |
| Gene ID (NCBI) | 3441 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05014 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
IFN-alpha 4 (IFN-α4 or IFNA4) belongs to the type I interferon family, a group of cytokines primarily produced in response to viral infections. As a key player in the innate immune response, IFN-α4 provides the body's first line of defense against pathogens by inducing an antiviral state in neighboring cells. Produced primarily by macrophages, IFN-α4 exerts its antiviral effects by binding to the interferon alpha receptor (IFNAR) on cell surfaces, thereby activating downstream immune signaling pathways . This binding triggers the expression of antiviral proteins, such as a protein kinase and an oligoadenylate synthetase, which inhibit viral RNA replication and enhance natural killer cell cytotoxicity against viruses.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IFN-alpha 4/IFNA4 antibody 98647-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |