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Validation Data Gallery

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  • Flow cytometry (FC) experiment of CHO cells using Anti-Human CCL24/Eotaxin 2 Rabbit Recombinant Anti (98476-3-RR)

    FC experiment of CHO using 98476-3-RR

    1x10^6 CHO-K1 cells (left) or CCL24/Eotaxin 2-transfected CHO-K1 cells (right) were intracellularly stained with 0.25 ug Anti-Human CCL24/Eotaxin 2 Rabbit RecAb (98476-3-RR, Clone: 243063C9) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Flow cytometry (FC) experiment of CHO cells using Anti-Human CCL24/Eotaxin 2 Rabbit Recombinant Anti (98476-3-RR)

    FC experiment of CHO using 98476-3-RR

    1x10^6 CHO cells (left) or CCL24/Eotaxin 2-transfected CHO cells (right) were intracellularly stained with 0.25 ug Anti-Human CCL24/Eotaxin 2 Rabbit RecAb (98476-3-RR, Clone: 243063C9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Anti-Human CCL24/Eotaxin 2 Rabbit Recombinant Antibody

CCL24/Eotaxin 2 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98476-3-RR
Clone No.243063C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CCL24, Eotaxin 2, C C motif chemokine 24, C-C motif chemokine 24, CCL 24

Formulation:  PBS, Azide
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inCHO cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98476-3-RR targets CCL24/Eotaxin 2 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0395

Product name: Recombinant Human CCL24 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 27-119 aa of NM_002991

Sequence: VVIPSPCCMFFVSKRIPENRVVSYQLSSRSTCLKAGVIFTTKKGQQFCGDPKQEWVQRYMKNLDAKQKKASPRARAVAVKGPVQRYPGNQTTC

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 24
Calculated molecular weight13kd
GenBank accession numberNM_002991
Gene Symbol CCL24/Eotaxin 2
Gene ID (NCBI) 6369
RRIDAB_3746106
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00175
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Chemokine CCL24 is the second member of eotaxins, a group of eosinophils' selectively chemoattractants. CCL24 is constitutively expressed in fibroblasts, epithelial cells, macrophages. CCL24 has only one receptor CCR3, which mainly presents on eosinophils and was also found on basophils, monocytes, Th2 lymphocytes, epithelial cells and airway smooth muscles. CCL24 displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes.

Protocols

Product Specific Protocols
FC protocol for CCL24/Eotaxin 2 antibody 98476-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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