Validation Data Gallery
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98339-3-RR targets KIR2DS4/CD158i in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg4353 Product name: Recombinant Human KIR2DS4 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 22-245 aa of NM_012314.6 Sequence: QEGVHRKPSFLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNNTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDPLDMVIIGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAVRSINGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLH 相同性解析による交差性が予測される生物種 |
| Full Name | killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4 |
| Calculated molecular weight | 34 kDa |
| GenBank accession number | NM_012314.6 |
| Gene Symbol | KIR2DS4 |
| Gene ID (NCBI) | 3809 |
| RRID | AB_3745989 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43632 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
KIR2DS4 (also known as CD158i) is a member of the killer cell immunoglobulin-like receptor (KIR) family. KIRs are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. KIRs are mainly involved in inhibiting NK killing (inhibitory KIRs) via interaction with MHC class I molecules. KIR2DS4 has been reported to be an activating KIR and recognize the HLA-Cw4 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for KIR2DS4/CD158i antibody 98339-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |