Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, L02 cells, HepG2 cells, HCT 116 cells, SW480 cells, mouse small intestine tissue, mouse colon tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
89174-1-RR targets TCF4/TCF7L2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4729 Product name: Recombinant human TCF7L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-355 aa of BC032656 Sequence: MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQ 相同性解析による交差性が予測される生物種 |
| Full Name | transcription factor 7-like 2 (T-cell specific, HMG-box) |
| Calculated molecular weight | 619 aa, 68 kDa |
| Observed molecular weight | 60, 75 kDa |
| GenBank accession number | BC032656 |
| Gene Symbol | TCF4 |
| Gene ID (NCBI) | 6934 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NQB0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TCF7L2 encodes an HMG-box transcription factor and key effector of canonical Wnt/β-catenin signaling, regulating development and tissue homeostasis. It is the strongest genetic susceptibility locus for type 2 diabetes. Alternative splicing produces functionally distinct isoforms, and TCF7L2 variants have also been linked to Crohn's disease and cancer, indicating pleiotropic roles in disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TCF4/TCF7L2 antibody 89174-1-RR | Download protocol |
| IP protocol for TCF4/TCF7L2 antibody 89174-1-RR | Download protocol |
| WB protocol for TCF4/TCF7L2 antibody 89174-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |