×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    WB analysis using 88615-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    WB analysis of mouse cerebellum using 88615-1-RR

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    WB analysis of pig brain using 88615-1-RR

    pig brain tissue were subjected to SDS PAGE followed by western blot with 88615-1-RR (Calbindin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    IHC staining of rat cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    IHC staining of rat cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    IHC staining of mouse cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    IHC staining of mouse cerebellum using 88615-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 88615-1-RR (Calbindin-D28k antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Calbindin-D28k Recombinant monoclonal antibody (88615-1-RR)

    IF Staining of mouse cerebellum using 88615-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Calbindin-D28k antibody (88615-1-RR, Clone: 261340A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 88615-1-RR

    Affinity of 88615-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88615-1-RR against Human Calbindin-D28k were performed. The affinity constant is 2.13 nM.

Calbindin-D28k Recombinant monoclonal antibody

Calbindin-D28k Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 88615-1-RR
Clone No.261340A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

CALB1, Calbindin, CALB, calbindin 1, 28kDa, Calbindin D28

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, pig brain tissue, rat brain tissue, fetal human brain tissue
Positive IHC detected inrat cerebellum tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88615-1-RR targets Calbindin-D28k in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5861

Product name: Recombinant human Calbindin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 110-261 aa of BC006478

Sequence: YDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMALSDGGKLYRTDLALILCAGDN

相同性解析による交差性が予測される生物種
Full Name calbindin 1, 28kDa
Calculated molecular weight 30 kDa
Observed molecular weight28 kDa
GenBank accession numberBC006478
Gene Symbol Calbindin
Gene ID (NCBI) 793
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05937
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Protocols

Product Specific Protocols
IF protocol for Calbindin-D28k antibody 88615-1-RRDownload protocol
IHC protocol for Calbindin-D28k antibody 88615-1-RRDownload protocol
WB protocol for Calbindin-D28k antibody 88615-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...