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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATXN10 Recombinant monoclonal antibody (88414-1-RR)

    WB analysis using 88414-1-RR (same clone as 88414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88414-1-RR (ATXN10 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATXN10 Recombinant monoclonal antibody (88414-1-RR)

    WB analysis using 88414-1-RR (same clone as 88414-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88414-1-RR (ATXN10 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88414-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03536-2

    Sandwich ELISA sample test of MP03536-2

    The mean ATXN10 concentration was determined to be 81.1 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load, 17.3 ng/mL in U-251 cell extract based on a 1.5 mg/mL extract load and 18.6 ng/mL in U2OS cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03536-2

    Sandwich ELISA standard curve of MP03536-2

    Sandwich ELISA standard curve of MP03536-2, Human ATXN10 Recombinant Matched Antibody Pair - PBS only. 88414-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8259. 88414-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Cytometric bead array sample test of MP03536-1

    Cytometric bead array sample test of MP03536-1

    The mean ATXN10 concentration was determined to be 80.7 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 13.9 ng/mL in U2OS cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03536-1

    Cytometric bead array standard curve of MP03536-1

    Cytometric bead array standard curve of MP03536-1, ATXN10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88414-1-PBS. Detection antibody: 88414-2-PBS. Standard: Ag8259. Range: 0.156-20 ng/mL.

ATXN10 Recombinant monoclonal antibody, PBS Only (Capture)

ATXN10 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 88414-1-PBS
Clone No.260934C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

ataxin 10, Ataxin-10, E46L, SCA10

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88414-1-PBS targets ATXN10 as part of a matched antibody pair:

MP03536-1: 88414-1-PBS capture and 88414-2-PBS detection (validated in Cytometric bead array)

MP03536-2: 88414-1-PBS capture and 88414-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8259

Product name: Recombinant human ATXN10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 120-475 aa of BC007508

Sequence: ELRVEQESLLTAFRCGLQFLGNIASRNEDSQSIVWVHAFPELFLSCLNHPDKKIVAYSSMILFTSLNHERMKELEENLNIAIDVIDAYQKHPESEWPFLIITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDEPLTKDDIPVFLRHAELIASTFVDQCKTVLKLASEEPPDDEEALATIRLLDVLCEMTVNTELLGYLQVFPGLLERVIDLLRVIHVAGKETTNIFSNCGCVRAEGDISNVANGFKSHLIRLIGNLCYKNKDNQDKVNELDGIPLILDNCNISDSNPFLTQWVIYAIRNLTEDNSQNQDLIAKMEEQGLADASLLKKVGFEVEKKGEKLILKSTRDTPKP

相同性解析による交差性が予測される生物種
Full Name ataxin 10
Calculated molecular weight 53 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC007508
Gene Symbol ATXN10
Gene ID (NCBI) 25814
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UBB4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

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