Various lysates were subjected to SDS PAGE followed by western blot with 88400-2-RR (CIRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88400-2-PBS in a different storage buffer formulation.

The mean CIRBP concentration was determined to be 41.78 ng/mL in U-251 cell extract based on a 1.20 mg/mL extract load, 48.22 ng/mL in MCF-7 cell extract based on a 1.50 mg/mL extract load and 19.36 ng/mL in U2OS cell extract based on a 1.20 mg/mL extract load.

Sandwich ELISA standard curve of MP03512-1, Human CIRBP Recombinant Matched Antibody Pair - PBS only. 88400-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0280. 88400-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

The mean CIRBP concentration was determined to be 94.4 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 48.3 ng/mL in RT-4 cell extract based on a 1.2 mg/mL extract load, 14.2 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load.

Cytometric bead array standard curve of MP03512-1, CIRBP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88400-3-PBS. Detection antibody: 88400-2-PBS. Standard: Ag0280. Range: 1.562-200 ng/mL.