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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Sam68 Recombinant monoclonal antibody (88301-1-RR)

    WB analysis using 88301-1-RR (same clone as 88301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88301-1-RR (Sam68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88301-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03453-2

    Sandwich ELISA sample test of MP03453-2

    The mean Sam68 concentration was determined to be 17.46 ng/mL in A2780 cell extract based on a 1.2 mg/mL extract load, 6.52 ng/mL in Caco-2 cell extract based on a 1.2 mg/mL extract load and 683.88 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03453-2

    Sandwich ELISA standard curve of MP03453-2

    Sandwich ELISA standard curve of MP03453-2, Human Sam68 Recombinant Matched Antibody Pair - PBS only. 88301-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0235. 88301-3-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Sam68 Recombinant monoclonal antibody (88301-1-RR)

    IF Staining of HepG2 using 88301-1-RR (same clone as 88301-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam68 antibody (88301-1-RR, Clone: 260478D3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88301-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03453-1

    Cytometric bead array sample test of MP03453-1

    The mean Sam68 concentration was determined to be 1791.9 ng/mL in HEK-293 cell extract based on a 1.0 mg/mL extract load, 7.8 ng/mL in Caco-2 cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP03453-1

    Cytometric bead array standard curve of MP03453-1

    Cytometric bead array standard curve of MP03453-1, Sam68 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88301-2-PBS. Detection antibody: 88301-1-PBS. Standard: Ag0235. Range: 0.625-80 ng/mL.

Sam68 Recombinant monoclonal antibody, PBS Only (Capture/Detector)

Sam68 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 88301-1-PBS
Clone No.260478D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

KHDRBS1, GAP-associated tyrosine phosphoprotein p62, KH domain-containing, RNA-binding, signal transduction-associated protein 1, KHDRBS 1, p21 Ras GTPase-activating protein-associated p62

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88301-1-PBS targets Sam68 as part of a matched antibody pair:

MP03453-1: 88301-2-PBS capture and 88301-1-PBS detection (validated in Cytometric bead array)

MP03453-2: 88301-1-PBS capture and 88301-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0235

Product name: Recombinant human Sam68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 201-442 aa of BC000717

Sequence: GSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPSRGRGVPVRGRGAAPPPPPVPRGRGVGPPRGALVRGTPVRGAITRGATVTRGVPPPPTVRGAPAPRARTAGIQRIPLPPPPAPETYEEYGYDDTYAEQSYEGYEGYYSQSQGDSEYYDYGHGEVQDSYEAYGQDDWNGTRPSLKAPPARPVKGAYREHPYGR

相同性解析による交差性が予測される生物種
Full Name KH domain containing, RNA binding, signal transduction associated 1
Calculated molecular weight 48 kDa
Observed molecular weight68 kDa
GenBank accession numberBC000717
Gene Symbol Sam68
Gene ID (NCBI) 10657
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ07666
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and INS receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP.

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