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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNFRSF6B Recombinant monoclonal antibody (87840-1-RR)

    WB analysis using 87840-1-RR (same clone as 87840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87840-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ACHN cells using TNFRSF6B Recombinant monoclonal antibody (87840-1-RR)

    WB analysis of ACHN using 87840-1-RR (same clone as 87840-1-PBS)

    ACHN cells were subjected to SDS PAGE followed by western blot with 87840-1-RR (TNFRSF6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87840-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03265-1

    Sandwich ELISA sample test of MP03265-1

    SW 480 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human DcR3/TNFRSF6B, and measured 29.05 ng/mL.

  • Sandwich ELISA sample test of MP03265-1

    Sandwich ELISA sample test of MP03265-1

    Plasma of five individual healthy human donors was measured. The human DcR3/TNFRSF6B concentration of detected samples was determined to be 2.47 ng/mL with a range of 0.38 - 9.39 ng/mL.

  • Sandwich ELISA standard curve of MP03265-1

    Sandwich ELISA standard curve of MP03265-1

    Sandwich ELISA standard curve of MP03265-1, Human DcR3/TNFRSF6B Recombinant Matched Antibody Pair - PBS only. 87840-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3110. 87840-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Affinity of 87840-1-RR

    Affinity of 87840-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87840-1-RR against Mouse TNFRSF6B were performed. The affinity constant is 0.131 nM.

TNFRSF6B Recombinant monoclonal antibody, PBS Only (Detector)

TNFRSF6B Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 87840-1-PBS
Clone No.253457A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

DcR3, Decoy receptor 3, Decoy receptor for Fas ligand, M68, TR6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87840-1-PBS targets TNFRSF6B as part of a matched antibody pair:

MP03265-1: 87840-2-PBS capture and 87840-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3110

Product name: Recombinant human TNFRSF6B protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 30-300 aa of NM_003823.3

Sequence: VAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 6b, decoy
Calculated molecular weight33 kDa
Observed molecular weight35 kDa
GenBank accession numberNM_003823.3
Gene Symbol TNFRSF6B
Gene ID (NCBI) 8771
RRIDAB_3745781
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95407
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The tumor necrosis factor receptor super-family member 6b (TNFRSF6B) is over-expressed in various human cancers. TNFRSF6B mRNA and protein expression in the tumor tissues negatively correlated with apoptosis. Cell proliferation was decreased, cell cycle was arrested in G1/S-phase, apoptosis was increased, and migration ability was inhibited by anti-TNFRSF6B McAb in vitro. Anti-TNFRSF6B McAb could be useful to suppress proliferation and induce apoptosis in HCC. Thus, TNFRSF6B might be a critical, targeted therapy strategy for HCC (PMID: 20591579).

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