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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    WB analysis using 87782-2-RR (same clone as 87782-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87782-2-RR (Claudin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    WB analysis of HT-29 using 87782-2-RR (same clone as 87782-2-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 87782-2-RR (Claudin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    WB analysis using 87782-2-RR (same clone as 87782-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87782-2-RR (Claudin 1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03201-1

    Sandwich ELISA sample test of MP03201-1

    The mean Claudin 1 concentration was determined to be 67.6 ng/mL in HUVEC cell extract based on a 1.5 mg/mL extract load and 5.0 ng/mL in MDA-MB-231 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03201-1

    Sandwich ELISA standard curve of MP03201-1

    Sandwich ELISA standard curve of MP03201-1, Human Claudin 1 Recombinant Matched Antibody Pair - PBS only. 87782-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag29269. 87782-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL.

  • Immunohistochemistry (IHC) staining of human skin tissue using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    IHC staining of human skin using 87782-2-RR (same clone as 87782-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87782-2-RR (Claudin 1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    IHC staining of human skin using 87782-2-RR (same clone as 87782-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87782-2-RR (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat colon tissue using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    IF Staining of rat colon using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    IF Staining of mouse colon using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    IF Staining of HaCaT using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HaCaT cells using Claudin 1 Recombinant monoclonal antibody (87782-2-RR)

    IF Staining of HaCaT using 87782-2-RR (same clone as 87782-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HaCaT cells using Claudin 1 antibody (87782-2-RR, Clone: 253345A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87782-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03201-1

    Cytometric bead array sample test of MP03201-1

    The mean Claudin 1 concentration was determined to be 2.2 ng/mL in A431 cell extract based on a 1.5 mg/mL extract load, 15.2 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, HCT-116 cell extract were served as a negative control.

  • Cytometric bead array standard curve of MP03201-1

    Cytometric bead array standard curve of MP03201-1

    Cytometric bead array standard curve of MP03201-1, Claudin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87782-2-PBS. Detection antibody: 87782-1-PBS. Standard: Ag29269. Range: 0.312-40 ng/mL.

Claudin 1 Recombinant monoclonal antibody, PBS Only (Capture)

Claudin 1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 87782-2-PBS
Clone No.253345A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Caudin 1, CLDN1, Claudin-1, CLD1, ILVASC

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87782-2-PBS targets Claudin 1 as part of a matched antibody pair:

MP03201-1: 87782-2-PBS capture and 87782-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag29269

Product name: Recombinant human Claudin 1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 102-211 aa of BC012471

Sequence: MKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

相同性解析による交差性が予測される生物種
Full Name claudin 1
Calculated molecular weight 211 aa, 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC012471
Gene Symbol Claudin 1
Gene ID (NCBI) 9076
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95832
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 Claudins have been identified. They are small (20-27 kilodaltons (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.

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