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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TRAF3IP2 Recombinant monoclonal antibody (87596-1-RR)

    WB analysis of MCF-7 using 87596-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 87596-1-RR (TRAF3IP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TRAF3IP2 Recombinant monoclonal antibody (87596-1-RR)

    WB analysis of A549 using 87596-1-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 87596-1-RR (TRAF3IP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity of 87596-1-RR

    Affinity of 87596-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87596-1-RR against Human TRAF3IP2 were performed. The affinity constant is below 1 pM.

TRAF3IP2 Recombinant monoclonal antibody

TRAF3IP2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87596-1-RR
Clone No.252775C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ACT1, Adapter protein CIKS, C6orf2, C6orf4, C6orf5

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87596-1-RR targets TRAF3IP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag24840

Product name: Recombinant human TRAF3IP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC002823

Sequence: MNRSIPVEVDESEPYPSQLLKPIPEYSPEEESEPPAPNIRNMAPNSLSAPTMLHNSSGDFSQAHSTLKLANHQRPVSRQVTCLRTQVLEDSEDSFCRRHPGLGKAFPSGCSAVSEPASESVVGALPAEHQFSFMEKRNQWLVSQLSAASPDTGHDSDKSDQSLPNASADSLGGSQEMVQRPQPHRNRAGLDLPTIDTGYDSQPQDVLGIRQLERPLPLTSVCYPQDLPRPLRSREFPQFEPQRYPACAQMLPPNLSPHAPWNYHYHCPGSPDHQVPYGHDYPRAAYQQVIQPALPGQPLPGASVRGLHPVQKVILNYPSPWDQEERPAQRDCSFPGLPRHQDQPHHQPPN

相同性解析による交差性が予測される生物種
Full Name TRAF3 interacting protein 2
Calculated molecular weight 65 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC002823
Gene Symbol TRAF3IP2
Gene ID (NCBI) 10758
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAF3IP2 antibody 87596-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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