Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87577-1-RR targets CD296/ART1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6314 Product name: Recombinant mouse CD296/ART1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 23-290 aa of NM_009710.4 Sequence: QSYSISQLDIFSQETPLDMAPASFDDQYAGCLADMTAALPDLNHSEFQANKVYADGWAQANNQWQERRAWGSVWGSLPPSPPGFRDEHGVALLAYTANSPLHKEFNAAVREAGRSRAHYLHHFSFKTLHFLLTEALQLLRSHRSRGCQQVYRGVHGLRFRPAGPGATVRLGGFASASLKNVAAQQFGEDTFFGIWTCLGAPIRGYSFFPEEEEVLIPPFETFQVINTSRPTQGPARIYLRALGKRSTYNCEYIKEKKCRSGPCWLGSS 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosyltransferase 1 |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | NM_009710.4 |
| Gene Symbol | Art1 |
| Gene ID (NCBI) | 11870 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q60935 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD296 (ART1) is a GPI-anchored ecto-ADP-ribosyltransferase that catalyzes extracellular mono-ADP-ribosylation of cell-surface proteins. By modifying integrins and other membrane-associated targets, ART1 regulates cell adhesion, migration, and inflammatory signaling. Its overexpression in multiple cancers and involvement in airway and tissue remodeling highlight ART1 as a functional mediator at the interface of cell-surface modification and disease progression.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD296/ART1 antibody 87577-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |