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Tested Applications
| Positive WB detected in | IGF-1 treated MCF-7 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87525-1-RR targets Phospho-PFKFB2 (Ser483) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 |
| Calculated molecular weight | 58 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | NM_006212 |
| Gene Symbol | PFKFB2 |
| Gene ID (NCBI) | 5208 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60825 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Phospho-PFKFB2 (Ser483) is the active-site-switching modification of the heart-type bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2). Phosphorylation at serine 483, located in the C-terminal regulatory segment, is catalyzed mainly by Akt downstream of PI3K and is essential for maximal 2-kinase activity: it increases the synthesis of fructose-2,6-bisphosphate (F2,6BP), the most potent allosteric activator of 6-phosphofructo-1-kinase (PFK-1), thereby accelerating glycolytic flux and ATP production. (PMID: 34282152)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PFKFB2 (Ser483) antibody 87525-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |