Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87446-1-RR targets LYPD8 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg7518 Product name: Recombinant mouse Lypd8 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-255 aa of NM_001083884.1 Sequence: LNCTQCYTYNSTCDGQATECNEQSFSCVESSINSTLGGFLHVYQNKFCSASNCTENSTEVAFTVHLFDDQRYHFASQCCQGESCNATHSESGTQNVTDMQCMSCYGHNKTLCEEKPQKCYEGEQCVFIIAEMVNGSGRVELKGCSDISNSTCQFLSPGNTTVGEFVFKSVECTQPTEYTNSTTTIPPITNTSLTSVTRPGIKTSPASVTPQASMGTKASFTSSIFGSLLLLKLLF 相同性解析による交差性が予測される生物種 |
| Full Name | RIKEN cDNA 2210415F13 gene |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 75 kDa, 45 kDa |
| GenBank accession number | NM_001083884.1 |
| Gene Symbol | 2210415F13Rik |
| Gene ID (NCBI) | 70163 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9D7S0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ly6/PLAUR domain-containing protein 8 (LYPD8) is a highly glycosylated glycosylphosphatidylinositol-anchored protein, which is anchored to intestinal epithelial cells in the uppermost epithelial layer, is constitutively shed into the intestinal lumen and preferentially binds to flagellated bacteria from various genera (PMID: 27027293; 29259722). It has been reported that Lypd8 mediates the segregation of intestinal bacteria and epithelial cells in the colon, thereby preserving intestinal homeostasis (PMID: 27027293). The higher observed molecular weight of LYPD8 is 75 kDa, due to glycosylation modification (PMID: 29259722).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LYPD8 antibody 87446-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |