Validation Data Gallery
Tested Applications
| Positive WB detected in | PMA, LPS and Brefeldin A treated RAW 264.7 cells |
| Positive IP detected in | PMA, LPS and Brefeldin A treated NR8383 cells |
| Positive IF/ICC detected in | PMA, LPS and Brefeldin A treated NR8383 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87439-1-RR targets CCL4/MIP-1 beta in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3200 Product name: recombinant rat CCL4/MIP-1 beta protein Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 24-92 aa of NM_053858.1 Sequence: APIGSDPPTSCCFSYTSRKIHRNFVMDYYETSSLCSQPAVVFLTKKGRQICADPSEPWVNEYVNDLELN 相同性解析による交差性が予測される生物種 |
| Full Name | chemokine (C-C motif) ligand 4 |
| Calculated molecular weight | 10 kDa |
| Observed molecular weight | 10 kDa |
| GenBank accession number | NM_053858.1 |
| Gene Symbol | Ccl4 |
| Gene ID (NCBI) | 116637 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P50230 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CCL4 (C-C motif chemokine ligand 4), also known as macrophage inflammatory protein-1β (MIP-1β), is a pivotal chemokine that governs immune cell migration and activation. By binding primarily to its receptor CCR5, it directs the recruitment of monocytes, dendritic cells, natural killer (NK) cells, and T lymphocytes to sites of inflammation and infection. CCL4 plays a crucial role in antiviral defense, antitumor immunity, and inflammatory responses. However, dysregulated CCL4 signaling is implicated in the pathogenesis of chronic inflammatory diseases, autoimmune disorders, and cancer progression. Notably, due to its shared use of the CCR5 receptor with HIV-1, CCL4 also participates in HIV pathogenesis. This dual role as a mediator of both protective immunity and pathology positions CCL4 as a significant biomarker and a promising therapeutic target in immunology and medicine.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCL4/MIP-1 beta antibody 87439-1-RR | Download protocol |
| IP protocol for CCL4/MIP-1 beta antibody 87439-1-RR | Download protocol |
| WB protocol for CCL4/MIP-1 beta antibody 87439-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |