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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TAU(Thr217) Recombinant monoclonal antibod (87428-1-RR)

    WB analysis using 87428-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain（AD） using Phospho-TAU (Thr217) Recombinant monoclonal antibo (87428-1-RR)

    IHC staining of human brain（AD） using 87428-1-RR

    Immunohistochemical analysis of paraffin-embedded Normal human brain tissue (left) and human Alzheimer's disease brain(AD) slide (right) using 87428-1-RR (Phospho-TAU (Thr217) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human large intestine tissue using Phospho-TAU(Thr217) Recombinant monoclonal antibod (87428-1-RR)

    IHC staining of human large intestine using 87428-1-RR

    Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human large intestine tissue using Phospho-TAU(Thr217) Recombinant monoclonal antibod (87428-1-RR)

    IHC staining of human large intestine using 87428-1-RR

    Immunohistochemical analysis of paraffin-embedded human large intestine tissue slide using 87428-1-RR (Phospho-TAU(Thr217) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Dot Blot experiment of / using Phospho-TAU(Thr217) Recombinant monoclonal antibod (87428-1-RR)

    Dot Blot experiment of / using 87428-1-RR

    Dot blot analysis was used to confirm the specificity of 87428-1-RR,Phospho-TAU (Thr217) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (25ng) spotted is indicated next to each row.

Phospho-TAU (Thr217) Recombinant monoclonal antibody

Phospho-TAU (Thr217) Uni-rAb® Recombinant Antibody for WB, IHC, Dot Blot, ELISA
Cat No. 87428-1-RR
Clone No.252442E8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Dot Blot, ELISA

Synonyms

p Tau, p Tau (Thr217), p Tau Thr217, phos TAU, Phospho Tau

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse blood
Positive IHC detected inhuman brain (AD), human large intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive Dot Blot detected in/ /

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
DOT BLOTDOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87428-1-RR targets Phospho-TAU (Thr217) in WB, IHC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight60-80 kDa
GenBank accession numberBC000558
Gene Symbol TAU
Gene ID (NCBI) 4137
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10636
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tau plays a crucial role in promoting and stabilizing microtubule assembly within neuronal axons. Phospho-TAU(Thr217) has emerged as a highly specific early biomarker for Alzheimer's disease (AD), detectable in both CSF and blood, effectively distinguishing AD from other dementias. This site is primarily phosphorylated by CDK5 and GSK-3β. Beyond its diagnostic value, p-T217 hyperphosphorylation is directly linked to synaptic dysfunction, neuronal loss, and cognitive decline in AD pathogenesis.

Protocols

Product Specific Protocols
WB protocol for Phospho-TAU (Thr217) antibody 87428-1-RRDownload protocol
IHC protocol for Phospho-TAU (Thr217) antibody 87428-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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