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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD1e Recombinant monoclonal antibody (87398-1-RR)

    WB analysis of Jurkat using 87398-1-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 87398-1-RR (CD1e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD1e Recombinant monoclonal antibody (87398-1-RR)

    WB analysis using 87398-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87398-1-RR (CD1e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD1e Recombinant monoclonal antibody (87398-1-RR)

    IF Staining of Jurkat using 87398-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD1e antibody (87398-1-RR, Clone: 252400H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87398-1-RR

    Affinity of 87398-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87398-1-RR against Human CD1e were performed. The affinity constant is 2.15 nM.

CD1e Recombinant monoclonal antibody

CD1e Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87398-1-RR
Clone No.252400H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

hCD1e, R2, R2G1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87398-1-RR targets CD1e in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg9185

Product name: Recombinant human B2M-CD1E protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011182 of

Sequence: \

相同性解析による交差性が予測される生物種
Full Name CD1e
Observed molecular weight27 kDa, 30-45 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP15812
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1e molecule (CD1e, also known as R2 and CD1A) is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin (PMID: 10948205). The discoverers of the CD1 locus originally separated CD1 proteins into group 1 (CD1a, CD1b, and CD1c) and group 2 (CD1d), based on amino acid sequence homology (PMID: 2467814). CD1e is an important modulator of group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability and the generation and persistence of CD1-lipid complexes (PMID: 21844346). CD1e may exhibit varying degrees of molecular weight migration in immunoblotting due to differences in glycosylation modification levels. The molecular weight of non N-glycosylated CD1e is 27 kDa (PMID: 19196239).

Protocols

Product Specific Protocols
IF protocol for CD1e antibody 87398-1-RRDownload protocol
WB protocol for CD1e antibody 87398-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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