Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HeLa cells |
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG).
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87365-3-RR targets Phospho-PKA C (Thr198) in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | protein kinase, cAMP-dependent, catalytic, alpha |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 38-43 kDa |
| GenBank accession number | NM_002730 |
| Gene Symbol | PRKACA |
| Gene ID (NCBI) | 5566 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P17612 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein kinase A (PKA) is a 3′-5′-cyclic adenosine monophosphate (cAMP)-dependent kinase that is at the center of diverse biological functions in numerous systems. Inactive PKA is composed of two catalytic (PKA-C) and two regulatory (PKA-R) subunits. The binding of cAMP to the PKA-R subunits activates the enzyme, causing the release of PKA-C. Humans have three genes encoding PKA-C: PRKACA, PRKACB, and PRKACG, and two types of PKA-R's: type PKA-RI (PKA-Riα and PKA-Riβ) and type PKA-RII (PKA-RIIα and PKA-RIIβ).(PMID: 36431013, PMID: 39592424)
This antibody recognizes the phosphorylation at the Thr198 site of PKA-C (including PRKACA, PRKACB, and PRKACG). After removing methionine, the site is also called Thr197.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PKA C (Thr198) antibody 87365-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |