Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, U2OS cells, mouse testis tissue, rat testis tissue, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87297-1-RR targets DTD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag11510 Product name: Recombinant human DTD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC045167 Sequence: MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFNLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP 相同性解析による交差性が予測される生物種 |
| Full Name | D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) |
| Calculated molecular weight | 209 aa, 23 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC045167 |
| Gene Symbol | DTD1 |
| Gene ID (NCBI) | 92675 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8TEA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DTD1 (D-aminoacyl-tRNA deacylase 1) is a crucial enzyme that ensures the fidelity of protein synthesis by maintaining L-homochirality. It functions as a proofreading factor that hydrolyzes mischarged D-aminoacyl-tRNAs into free D-amino acids and tRNA molecules, preventing the incorporation of D-amino acids into polypeptide chains. DTD1 also plays a significant part in the initiation of DNA replication by facilitating the loading of the CDC45 complex onto pre-replication sites. (PMID: 18700836;27200345)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DTD1 antibody 87297-1-RR | Download protocol |
| WB protocol for DTD1 antibody 87297-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |