Validation Data Gallery
Tested Applications
| Positive WB detected in | human Neuregulin-1 treated T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87133-1-RR targets Phospho-ErbB3/HER3 (Tyr1197) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) |
| Calculated molecular weight | 148 kDa |
| Observed molecular weight | 185 kDa |
| GenBank accession number | NM_001982 |
| Gene Symbol | ERBB3 |
| Gene ID (NCBI) | 2065 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21860 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. Phospho-ErbB3/HER3 (Tyr1197) is a phosphorylated form of the epidermal growth factor receptor 3 (EGFR3), also known as HER3. HER3 lacks tyrosine kinase activity itself, but becomes phosphorylated at tyrosine residues after forming heterodimers with other ErbB proteins such as HER2. The HER2/HER3 dimer is a potent activator of growth signaling pathways including MAPK. This phosphorylation event is involved in signal transduction pathways related to cell growth, proliferation, and survival. (PMID: 35625984)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ErbB3/HER3 (Tyr1197) antibody 87133-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |