Validation Data Gallery
Tested Applications
| Positive WB detected in | SW480 cells, K-562 cells, HEK-293 cells, HepG2 cells, PANC-1 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87117-4-RR targets MINPP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34998 Product name: Recombinant human MINPP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC032504 Sequence: MLRAPGCLLRTSVAPAAALAAALLSSLARCSLLEPRDPVASSLSPYFGTKTRYEDVNPVLLSGPEAPWRDPELLEGTCTPVQLVALIRHGTRYPTVKQIRKLRQLHGLLQARGSRDGGASSTGSRDLGAALADWPLWYADWMDGQLVEKGRQDMRQLALRLASLFPALFSRENYGRLRLITSSKHRCMDSSAAFLQGLWQHYHPGLPPPDVADMEFGPPTVNDKLMRFFDHCEKFLTEVEKNATALYHVEAFKTGPEMQNILKKVAATLQVPVNDLNADLIQVAFFTCSFDLAIKGVKSPWCDVFDIDDAKVLEYLNDLKQYWKRGYGYTINSRSSCTL 相同性解析による交差性が予測される生物種 |
| Full Name | multiple inositol polyphosphate histidine phosphatase, 1 |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC032504 |
| Gene Symbol | MINPP1 |
| Gene ID (NCBI) | 9562 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UNW1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MINPP1 (multiple inositol-polyphosphate phosphatase 1), also known as MIPP and MINPP2, is an enzyme that catalyzes the removal of the 3-phosphate group from inositol phosphate substrates. It plays a crucial role in the metabolism of inositol polyphosphates, which are important second messengers in cellular signaling pathways. MINPP1 has been implicated in various diseases, including certain types of cancer, where its expression levels may be associated with tumor progression and differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MINPP1 antibody 87117-4-RR | Download protocol |
| WB protocol for MINPP1 antibody 87117-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |