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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Apolipoprotein CIII Recombinant monoclonal antibod (87100-1-RR)

    WB analysis using 87100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87100-1-RR (Apolipoprotein CIII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Apolipoprotein CIII Recombinant monoclonal antibod (87100-1-RR)

    WB analysis using 87100-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87100-1-RR (Apolipoprotein CIII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using Apolipoprotein CIII Recombinant monoclonal antibod (87100-1-RR)

    IF Staining of L02 using 87100-1-RR

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using Apolipoprotein CIII antibody (87100-1-RR, Clone: 252153H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 87100-1-RR

    Affinity and Kinetic Characterization of 87100-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87100-1-RR against Human Apolipoprotein CIII were performed. The affinity constant is below 1 pM.

Apolipoprotein CIII Recombinant monoclonal antibody

Apolipoprotein CIII Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87100-1-RR
Clone No.252153H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

APOC3, Apo CIII, ApoC III, APOCIII, Apo-CIII

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma, human serum
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87100-1-RR targets Apolipoprotein CIII in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3387

Product name: recombinant human APOC3 protein

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 21-99 aa of NM_000040

Sequence: SEAEDASLLSFMQGYMKHATKTAKDALSSVQESQVAQQARGWVTDGFSSLKDYWSTVKDKFSEFWDLDPEVRPTSAVAA

相同性解析による交差性が予測される生物種
Full Name apolipoprotein C-III
Calculated molecular weight 11 kDa
Observed molecular weight11 kDa
GenBank accession numberNM_000040
Gene Symbol Apolipoprotein CIII
Gene ID (NCBI) 345
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02656
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apolipoprotein CIII (apoCIII) is the most abundant C-apolipoprotein in human and is found on very low density lipoproteins, chylomicrons, and high density lipoproteins (HDL) (PMID: 18408013). ApoCIII inhibits lipoprotein lipase as well as binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors (PMID: 26228667). ApoC-III has 3 actions that can impair plasma lipoprotein metabolism and lead to hypertriglyceridemia (PMID: 24941081).

Protocols

Product Specific Protocols
IF protocol for Apolipoprotein CIII antibody 87100-1-RRDownload protocol
WB protocol for Apolipoprotein CIII antibody 87100-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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