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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCL5 Recombinant monoclonal antibody (87060-4-RR)

    WB analysis using 87060-4-RR

    LPS, PMA, and Brefeldin A-treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 87060-4-RR (CCL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CCL5 Recombinant monoclonal antibody (87060-4-RR)

    IHC staining of mouse spleen using 87060-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87060-4-RR (CCL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CCL5 Recombinant monoclonal antibody (87060-4-RR)

    IHC staining of mouse spleen using 87060-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87060-4-RR (CCL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CCL5 Recombinant monoclonal antibody (87060-4-RR)

    IHC staining of mouse colon using 87060-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87060-4-RR (CCL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CCL5 Recombinant monoclonal antibody (87060-4-RR)

    IHC staining of mouse colon using 87060-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 87060-4-RR (CCL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCL5 Recombinant monoclonal antibody

CCL5 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87060-4-RR
Clone No.250841C2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

C-C motif chemokine 5, MuRantes, RANTES, Scya5, SIS-delta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated RAW 264.7 cells
Positive IHC detected inmouse spleen tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87060-4-RR targets CCL5 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2253

Product name: Recombinant Mouse CCL5 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-91 aa of Q5XZF2

Sequence: SPYGSDTTPCCFAYLSLALPRAHVKEYFYTSSKCSNLAVVFVTRRNRQVCANPEKKWVQEYINYLEMS

相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 5
Calculated molecular weight10kd
Observed molecular weight8 kDa
GenBank accession numberQ5XZF2
Gene Symbol Ccl5
Gene ID (NCBI) 20304
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP30882
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCL5 (C-C motif chemokine ligand 5), also known as RANTES, is a key inflammatory chemokine that attracts immune cells like T cells, monocytes, and eosinophils to sites of infection or inflammation, playing vital roles in immunity, viral defense (including HIV), and sometimes cancer progression, by binding to receptors like CCR1, CCR3, and CCR5 to signal cell movement and activation, with inhibitors being researched for therapeutic use.

Protocols

Product Specific Protocols
IHC protocol for CCL5 antibody 87060-4-RRDownload protocol
WB protocol for CCL5 antibody 87060-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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