Various lysates were subjected to SDS PAGE followed by western blot with 87058-2-RR (PDLIM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87058-2-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 87058-2-RR (PDLIM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87058-2-PBS in a different storage buffer formulation.

human heart tissue were subjected to SDS PAGE followed by western blot with 87058-2-RR (PDLIM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87058-2-PBS in a different storage buffer formulation.

The mean PDLIM3 concentration was determined to be 321.00 ng/mL in mouse skeletal muscle extract based on a 4.10 mg/mL extract load, 29.50 ng/mL in mouse heart extract based on a 5.40 mg/mL extract load, 6.40 ng/mL in mouse liver extract based on a 8.40 mg/mL extract load and 14.40 ng/mL in mouse kidney extract based on a 8.4 mg/mL extract load.

Sandwich ELISA standard curve of MP02852-1, Human PDLIM3 Recombinant Matched Antibody Pair - PBS only. 87058-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3934. 87058-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

The mean PDLIM3 concentration was determined to be 495.6 ng/mL in mouse spleen tissue extract based on a 6.4 mg/mL extract load.

Cytometric bead array standard curve of MP02852-1, PDLIM3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87058-2-PBS. Detection antibody: 87058-1-PBS. Standard: Ag3934. Range: 0.156-20 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 87058-2-RR against Human MCM6 were performed. The affinity constant is 0.242 nM.