• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Integrin alpha 1 Recombinant monoclonal antibody (87056-2-RR)

    WB analysis using 87056-2-RR (same clone as 87056-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87056-2-RR (Integrin alpha-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Integrin alpha 1 Recombinant monoclonal antibody (87056-2-RR)

    WB analysis of human placenta using 87056-2-RR (same clone as 87056-2-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 87056-2-RR (Integrin alpha-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02854-2

    Sandwich ELISA sample test of MP02854-2

    The mean Integrin alpha 1 concentration was determined to be 241.89 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load, 523.16 ng/mL in HuH-7 cell extract based on a 1.20 mg/mL extract load, 15.42 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load and 17.42 ng/mL in U-937 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA sample test of MP02854-2

    Sandwich ELISA sample test of MP02854-2

    Plasma of eight individual healthy human donors was measured. The Integrin alpha 1 concentration of detected samples was determined to be 84.94 ng/mL with a range of 50.93-128.02 ng/mL.

  • Sandwich ELISA standard curve of MP02854-2

    Sandwich ELISA standard curve of MP02854-2

    Sandwich ELISA standard curve of MP02854-2, Human Integrin alpha 1 Recombinant Matched Antibody Pair - PBS only. 87056-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2089. 87056-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Integrin alpha 1 Recombinant monoclonal antibody (87056-2-RR)

    IF Staining of HeLa using 87056-2-RR (same clone as 87056-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Integrin alpha 1 antibody (87056-2-RR, Clone: 251984B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02854-1

    Cytometric bead array sample test of MP02854-1

    The mean Integrin alpha-1 concentration was determined to be 51.4 ng/mL in human plasma.

  • Cytometric bead array standard curve of MP02854-1

    Cytometric bead array standard curve of MP02854-1

    Cytometric bead array standard curve of MP02854-1, Integrin alpha-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87056-2-PBS. Detection antibody: 87056-1-PBS. Standard: Eg2089. Range: 0.781-100 ng/mL.

  • Affinity of 87056-2-PBS

    Affinity of 87056-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 87056-2-PBS against Human Integrin alpha-1 was performed. The affinity constant is 1.16 nM.

  • Affinity of 87056-2-RR

    Affinity of 87056-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87056-2-RR against Human Integrin alpha 1 were performed. The affinity constant is 0.468 nM.

Integrin alpha 1 Recombinant monoclonal antibody, PBS Only (Capture)

Integrin alpha 1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 87056-2-PBS
Clone No.251984B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

ITGA1, CD49 antigen-like family member A, CD49a, integrin, alpha 1, Laminin and collagen receptor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87056-2-PBS targets Integrin alpha 1 as part of a matched antibody pair:

MP02854-1: 87056-2-PBS capture and 87056-1-PBS detection (validated in Cytometric bead array)

MP02854-2: 87056-2-PBS capture and 87056-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2089

Product name: recombinant human Integrin alpha-1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 29-1141 aa of BC137121

Sequence: FNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKNRTGDVYKCPVGRGESLPCVKLDLPVNTSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKASQIIIPRNTTFNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYFGSILTTTDIDKDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTTENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHGGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINATVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQDSVRITLDFNLTDPENGPVLDDSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVP

相同性解析による交差性が予測される生物種
Full Name integrin, alpha 1
Calculated molecular weight 1179 aa, 131 kDa
Observed molecular weight200 kDa
GenBank accession numberBC137121
Gene Symbol Integrin alpha 1
Gene ID (NCBI) 3672
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP56199
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

||
New chat Able
正在加载，请稍候...