Various lysates were subjected to SDS PAGE followed by western blot with 87035-1-RR (SMAD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87035-1-PBS in a different storage buffer formulation.

The mean SMAD3 concentration was determined to be 503.2 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load, 253.4 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, 709.6 ng/mL in SH-SY5Y cell extract based on a 1.2 mg/mL extract load, 646.7 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load and 750.4 ng/mL in NIH/3T3 cell extract based on a 1.2 mg/mL extract load.

Serum of sixteen individual healthy human donors was measured. The SMAD3 concentration of detected samples was determined to be 128.2 pg/mL with a range of 31.3-281.8 pg/mL.

Sandwich ELISA standard curve of MP02853-2, Human SMAD3 Recombinant Matched Antibody Pair - PBS only. 87035-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32623. 87035-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

Immunohistochemical analysis of paraffin-embedded mouse prostate tissue slide using 87035-1-RR (SMAD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87035-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SMAD3 antibody (87035-1-RR, Clone: 251981H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87035-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 87035-1-RR against Human SMAD3 were performed. The affinity constant is 1.26 nM.