Various lysates were subjected to SDS PAGE followed by western blot with 86968-1-RR (Syntenin-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86968-1-PBS in a different storage buffer formulation.

The mean Syntenin-1 concentration was determined to be 8,459.69 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 4,141.00 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load, 6,144.14 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 3,765.64 ng/mL in HCT 116 cell extract based on a 1.3 mg/mL extract load and 339.31 ng/mL in PC-12 cell extract based on a 1.4 mg/mL extract load.

Sandwich ELISA standard curve of MP02969-1, Human Syntenin-1 Recombinant Matched Antibody Pair - PBS only. 86968-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag18078. 86968-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL

IP result of anti-Syntenin-1 (IP:86968-1-RR, 4ug; Detection:86968-1-RR 1:1000) with HeLa cells lysate 1480 ug. This data was developed using the same antibody clone with 86968-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86968-1-RR (Syntenin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86968-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Syntenin-1 antibody (86968-1-RR, Clone: 252107H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86968-1-PBS in a different storage buffer formulation.