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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP1R10 Recombinant monoclonal antibody (86888-1-RR)

    WB analysis using 86888-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86888-1-RR (PPP1R10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PPP1R10 Recombinant monoclonal antibody (86888-1-RR)

    IF Staining of HepG2 using 86888-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PPP1R10 antibody (86888-1-RR, Clone: 251508G3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PPP1R10 Recombinant monoclonal antibody

PPP1R10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86888-1-RR
Clone No.251508G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CAT53, FB19, MHC class I region proline-rich protein CAT53, p99, PNUTS

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86888-1-RR targets PPP1R10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag20091

Product name: Recombinant human PPP1R10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-318 aa of BC150310

Sequence: MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVGGYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDWMAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAK

相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 10
Calculated molecular weight 940 aa, 99 kDa
Observed molecular weight 99-110 kDa
GenBank accession numberBC150310
Gene Symbol PPP1R10
Gene ID (NCBI) 5514
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96QC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PPP1R10 antibody 86888-1-RRDownload protocol
WB protocol for PPP1R10 antibody 86888-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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