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Validation Data Gallery

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  • Sandwich ELISA sample test of MP02694-2

    Sandwich ELISA sample test of MP02694-2

    The mean IGF-II R/IGF2R concentration was determined to be 344.83 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 214.23 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP02694-2

    Sandwich ELISA sample test of MP02694-2

    Serum of four individual healthy human donors was measured. The human IGF-II R/IGF2R concentration of detected samples was determined to be 291.46 ng/mL with a range of 273.79 - 315.06 ng/mL.

  • Sandwich ELISA standard curve of MP02694-2

    Sandwich ELISA standard curve of MP02694-2

    Sandwich ELISA standard curve of MP02694-2, Human IGF-II R/IGF2R Recombinant Matched Antibody Pair - PBS only. 86821-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3488. 86821-4-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

  • Cytometric bead array sample test of MP02694-1

    Cytometric bead array sample test of MP02694-1

    The mean IGF-II R/IGF2R concentration was determined to be 248.2 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 74.7 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02694-1

    Cytometric bead array standard curve of MP02694-1

    Cytometric bead array standard curve of MP02694-1, IGF-II R/IGF2R Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86821-3-PBS. Detection antibody: 86821-2-PBS. Standard: Eg3488. Range: 1.562-200 ng/mL.

  • Affinity and Kinetic Characterization of 86821-3-PBS

    Affinity and Kinetic Characterization of 86821-3-PBS

    Biolayer interferometry (BLl) kinetic assays of 86821-3-PBS against Human IGF-II R/IGF2R were performed. The affinity constant is 1.16 nM.

IGF-II R/IGF2R Recombinant monoclonal antibody, PBS Only (Capture)

IGF-II R/IGF2R Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 86821-3-PBS
Clone No.251265C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

IGF2R, 300 kDa mannose 6-phosphate receptor, Cation-independent mannose-6-phosphate receptor, CD222, CI Man 6 P receptor

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86821-3-PBS targets IGF-II R/IGF2R as part of a matched antibody pair:

MP02694-1: 86821-3-PBS capture and 86821-2-PBS detection (validated in Cytometric bead array)

MP02694-2: 86821-3-PBS capture and 86821-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3488

Product name: recombinant human IGF-II R/IGF2R protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 41-1365 aa of NM_000876.4

Sequence: QAAPFPELCSYTWEAVDTKNNVLYKINICGSVDIVQCGPSSAVCMHDLKTRTYHSVGDSVLRSATRSLLEFNTTVSCDQQGTNHRVQSSIAFLCGKTLGTPEFVTATECVHYFEWRTTAACKKDIFKANKEVPCYVFDEELRKHDLNPLIKLSGAYLVDDSDPDTSLFINVCRDIDTLRDPGSQLRACPPGTAACLVRGHQAFDVGQPRDGLKLVRKDRLVLSYVREEAGKLDFCDGHSPAVTITFVCPSERREGTIPKLTAKSNCRYEIEWITEYACHRDYLESKTCSLSGEQQDVSIDLTPLAQSGGSSYISDGKEYLFYLNVCGETEIQFCNKKQAAVCQVKKSDTSQVKAAGRYHNQTLRYSDGDLTLIYFGGDECSSGFQRMSVINFECNKTAGNDGKGTPVFTGEVDCTYFFTWDTEYACVKEKEDLLCGATDGKKRYDLSALVRHAEPEQNWEAVDGSQTETEKKHFFINICHRVLQEGKARGCPEDAAVCAVDKNGSKNLGKFISSPMKEKGNIQLSYSDGDDCGHGKKIKTNITLVCKPGDLESAPVLRTSGEGGCFYEFEWHTAAACVLSKTEGENCTVFDSQAGFSFDLSPLTKKNGAYKVETKKYDFYINVCGPVSVSPCQPDSGACQVAKSDEKTWNLGLSNAKLSYYDGMIQLNYRGGTPYNNERHTPRATLITFLCDRDAGVGFPEYQEEDNSTYNFRWYTSYACPEEPLECVVTDPSTLEQYDLSSLAKSEGGLGGNWYAMDNSGEHVTWRKYYINVCRPLNPVPGCNRYASACQMKYEKDQGSFTEVVSISNLGMAKTGPVVEDSGSLLLEYVNGSACTTSDGRQTTYTTRIHLVCSRGRLNSHPIFSLNWECVVSFLWNTEAACPIQTTTDTDQACSIRDPNSGFVFNLNPLNSSQGYNVSGIGKIFMFNVCGTMPVCGTILGKPASGCEAETQTEELKNWKPARPVGIEKSLQLSTEGFITLTYKGPLSAKGTADAFIVRFVCNDDVYSGPLKFLHQDIDSGQGIRNTYFEFETALACVPSPVDCQVTDLAGNEYDLTGLSTVRKPWTAVDTSVDGRKRTFYLSVCNPLPYIPGCQGSAVGSCLVSEGNSWNLGVVQMSPQAAANGSLSIMYVNGDKCGNQRFSTRITFECAQISGSPAFQLQDGCEYVFIWRTVEACPVVRVEGDNCEVKDPRHGNLYDLKPLGLNDTIVSAGEYTYYFRVCGKLSSDVCPTSDKSKVVSSCQEKREPQGFHKVAGLLTQKLTYENGLLKMNFTGGDTCHKVYQRSTAIFFYCDRGTQRPVFLKETSDCSYLFEWRTQYACPPFD

相同性解析による交差性が予測される生物種
Full Name insulin-like growth factor 2 receptor
Calculated molecular weight274 kDa
GenBank accession numberNM_000876.4
Gene Symbol IGF2R
Gene ID (NCBI) 3482
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP11717
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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