Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, PC-3 cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86646-1-RR targets SCRN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5413 Product name: Recombinant human SCRN1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-414 aa of BC040492 Sequence: MAAAPPSYCFVAFPPRAKDGLVVFGKNSARPRDEVQEVVYFSAADHEPESKVECTYISIDQVPRTYAIMISRPAWLWGAEMGANEHGVCIANEAINTREPAAEIEALLGMDLVRLGLERGETAKEALDVIVSLLEEHGQGGNYFEDANSCHSFQSAYLIVDRDEAWVLETIGKYWAAEKVTEGVRCICSQLSLTTKMDAEHPELRSYAQSQGWWTGEGEFNFSEVFSPVEDHLDCGAGKDSLEKQEESITVQTMMNTLRDKASGVCIDSEFFLTTASGVSVLPQNRSSPCIHYFTGTHDPSRSIFKPFIFVDDVKLVPKTQSPCFGDDDPAKKEPRFQEKPDRRHELYKAHEWARAIIESDQEQGRKLRSTMLELEKQGLEAMEEILTSSEPLDPAEVGDLFYDCVDTEIKFFK 相同性解析による交差性が予測される生物種 |
| Full Name | secernin 1 |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC040492 |
| Gene Symbol | SCRN1 |
| Gene ID (NCBI) | 9805 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SCRN1 antibody 86646-1-RR | Download protocol |
| WB protocol for SCRN1 antibody 86646-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |