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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP153 Recombinant monoclonal antibody (86622-1-RR)

    WB analysis using 86622-1-RR (same clone as 86622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86622-1-RR (NUP153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02679-1

    Sandwich ELISA sample test of MP02679-1

    The mean NUP153 concentration was determined to be 30.07 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load, 97.51 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 33.07 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02679-1

    Sandwich ELISA standard curve of MP02679-1

    Sandwich ELISA standard curve of MP02679-1, Human NUP153 Recombinant Matched Antibody Pair - PBS only. 86622-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5519. 86622-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUP153 Recombinant monoclonal antibody (86622-1-RR)

    IF Staining of HeLa using 86622-1-RR (same clone as 86622-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUP153 antibody (86622-1-RR, Clone: 251519G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86622-1-RR

    Affinity and Kinetic Characterization of 86622-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86622-1-RR against Human NUP153 were performed. The affinity constant is 2.32 nM.

NUP153 Recombinant monoclonal antibody, PBS Only (Capture)

NUP153 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86622-1-PBS
Clone No.251519G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86622-1-PBS targets NUP153 as part of a matched antibody pair:

MP02679-1: 86622-1-PBS capture and 86622-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5519

Product name: Recombinant human NUP153 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1125-1475 aa of BC052965

Sequence: EEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPSEKESEQPAKATFAFGAQTSTTADQGAAKPVFSFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFGQSSNPVSSSAFGNTAESSTSQSLLFSQDSKLATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSSAGSSFVFGTGPSAPSASPAFGANQTPTFGQSQGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSSQPPVFGQQPSQSAFGSGTTPNSSSAFQFGSSTTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFNQSPAAFTVGSNGKNVFSSSGTSFSGRKIKTAVRRRK

相同性解析による交差性が予測される生物種
Full Name nucleoporin 153kDa
Calculated molecular weight 154 kDa
Observed molecular weight160-170 kDa
GenBank accession numberBC052965
Gene Symbol NUP153
Gene ID (NCBI) 9972
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP49790
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].

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