Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, mouse pancreas tissue, HepG2 cells, HuH-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86354-1-RR targets DUSP19 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3611 Product name: Recombinant human DUSP19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC035000 Sequence: MYSLNQEIKAFSRNNLRKQCTRVTTLTGKKIIETWKDARIHVVEEVEPSSGGGCGYVQDLSSDLQVGVIKPWLLLGSQDAAHDLDTLKKNKVTHILNVAYGVENAFLSDFTYKSISILDLPETNILSYFPECFEFIEEAKRKDGVVLVHCNAGVSRAAAIVIGFLMNSEQTSFTSAFSLVKNARPSICPNSGFMEQLRTYQEGKESNKCDRIQENSS 相同性解析による交差性が予測される生物種 |
| Full Name | dual specificity phosphatase 19 |
| Calculated molecular weight | 217 aa, 24 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC035000 |
| Gene Symbol | DUSP19 |
| Gene ID (NCBI) | 142679 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WTR2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DUSP19 (Dual-Specificity Phosphatase 19), also known as SKRP1, LMW-DSP3, or SAPK pathway-regulating phosphatase 1, is a low-molecular-weight member of the dual-specificity phosphatase family. It is encoded on human chromosome 2q32 and expressed in multiple tissues, with the highest levels found in the pancreas and heart. Structurally, DUSP19 contains a conserved catalytic domain that removes phosphate groups from both phospho-tyrosine and phospho-serine/threonine residues, enabling it to negatively or positively regulate MAPK signaling.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP19 antibody 86354-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |