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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B4 Recombinant monoclonal antibody (86334-2-RR)

    WB analysis using 86334-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86334-2-RR (HSD17B4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSD17B4 Recombinant monoclonal antibody (86334-2-RR)

    IF Staining of HeLa using 86334-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSD17B4 antibody (86334-2-RR, Clone: 251058E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86334-2-RR

    Affinity and Kinetic Characterization of 86334-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86334-2-RR against Human HSD17B4 were performed. The affinity constant is below 1 pM.

HSD17B4 Recombinant monoclonal antibody

HSD17B4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86334-2-RR
Clone No.251058E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

(3R)-hydroxyacyl-CoA dehydrogenase, 17 beta HSD 4, 17-beta-HSD 4, 17-beta-hydroxysteroid dehydrogenase 4, D bifunctional protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, HepG2 cells, mouse liver tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86334-2-RR targets HSD17B4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7165

Product name: Recombinant human HSD17B4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 436-736 aa of BC003098

Sequence: GSGVVIIMDVYSYSEKELICHNQFSLFLVGSGGFGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAKPVYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDLAPTSGTSAKTPSEGGKLQSTFVFEEIGRRLKDIGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKVYQGPAKGAADTTIILSDEDFMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL

相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 4
Calculated molecular weight 80 kDa
Observed molecular weight75 kDa, 45 kDa
GenBank accession numberBC003098
Gene Symbol HSD17B4
Gene ID (NCBI) 3295
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP51659
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

Protocols

Product Specific Protocols
IF protocol for HSD17B4 antibody 86334-2-RRDownload protocol
WB protocol for HSD17B4 antibody 86334-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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