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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD4 Recombinant antibody (86300-3-RR)

    WB analysis using 86300-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86300-3-RR (CD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD4 Recombinant monoclonal antibody (86300-3-RR)

    IHC staining of mouse spleen using 86300-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD4 Recombinant monoclonal antibody (86300-3-RR)

    IHC staining of mouse spleen using 86300-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD4 Recombinant monoclonal antibody (86300-3-RR)

    IHC staining of mouse thymus using 86300-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD4 Recombinant monoclonal antibody (86300-3-RR)

    IF Staining of mouse spleen using 86300-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse thymus tissue using CD4 Recombinant monoclonal antibody (86300-3-RR)

    IF Staining of mouse thymus using 86300-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse thymus tissue using CD4 Recombinant monoclonal antibody (86300-3-RR)

    IF Staining of mouse thymus using 86300-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD4 Recombinant monoclonal antibody

CD4 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86300-3-RR
Clone No.250263G12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse thymus tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue, mouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse thymus tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86300-3-RR targets CD4 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3847

Product name: Recombinant Mouse CD4 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 27-394 aa of NM_013488

Sequence: KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

相同性解析による交差性が予測される生物種
Full Name CD4 antigen
Calculated molecular weight51 kDa
Observed molecular weight55 kDa
GenBank accession numberNM_013488
Gene Symbol Cd4
Gene ID (NCBI) 12504
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06332
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
WB protocol for CD4 antibody 86300-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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