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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK12/CRKRS Recombinant antibody (86296-3-RR)

    WB analysis using 86296-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CDK12/CRKRS Recombinant antibody (86296-3-RR)

    WB analysis of NIH/3T3 using 86296-3-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CDK12/CRKRS Recombinant antibody (86296-3-RR)

    IF Staining of NIH/3T3 using 86296-3-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK12/CRKRS Recombinant antibody (86296-3-RR)

    IF Staining of HeLa using 86296-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86296-3-RR

    Affinity and Kinetic Characterization of 86296-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86296-3-RR against Human CDK12/CRKRS were performed. The affinity constant is 0.106 nM.

CDK12/CRKRS Recombinant monoclonal antibody

CDK12/CRKRS Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86296-3-RR
Clone No.250880E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CrkRS, CDC2 related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, CDC2-related protein kinase 7, CDK12

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293T cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86296-3-RR targets CDK12/CRKRS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25312

Product name: Recombinant human CRKRS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1300-1481 aa of BC150265

Sequence: EAEPPGHLPHEHQALRPMEYSTRPRPNRTYGNTDGPETGFSAIDTDERNSGPALTESLVQTLVKNRTFSGSLSHLGESSSYQGTGSVQFPGDQDLRFARVPLALHPVVGQPFLKAEGSSNSVVHAETKLQNYGELGPGTTGASSSGAGLHWGGPTQSSAYGKLYRGPTRVPPRGGRGRGVPY

相同性解析による交差性が予測される生物種
Full Name Cdc2-related kinase, arginine/serine-rich
Calculated molecular weight 1490 aa, 164 kDa
Observed molecular weight 180-200 kDa
GenBank accession numberBC150265
Gene Symbol CDK12
Gene ID (NCBI) 51755
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NYV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRKRS (Cdc2-related kinase, Arg/Ser), also named as cyclin-dependent kinase 12 (CKD12), belongs to CDC2/CDKX subfamily. CDK12 can form a heterodimeric complex with its activating partner, cyclin K, to regulate important cellular physiological processes that include transcriptional regulation, regulation of DNA damage repair genes such as BRCA1 and ATM, suppression of intronic polyadenylation, and susceptibility to PARP inhibitors (PMID: 30487607, 24240700, 31694772). CDK12 has 3 isoforms with the calculated molecular mass of 164/163/141 kDa, and the apparent molecular mass of 180-200 kDa due to some modifications (PMID 11683387, 22988298).

Protocols

Product Specific Protocols
IF protocol for CDK12/CRKRS antibody 86296-3-RRDownload protocol
WB protocol for CDK12/CRKRS antibody 86296-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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