Validation Data Gallery
Tested Applications
| Positive WB detected in | T-47D cells, mouse skin tissue, rat skin tissue, HeLa cells, MCF-7 cells, A431 cells, HaCaT cells |
| Positive IHC detected in | mouse esophagus tissue, human skin tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skin tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86277-2-RR targets Cytokeratin 39 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | keratin 39 |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC157887 |
| Gene Symbol | KRT39 |
| Gene ID (NCBI) | 390792 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6A163 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytokeratin 39 (CK-39) is a member of the cytokeratin family, a group of intermediate filament proteins primarily expressed in epithelial cells. Cytokeratins are classified into Type I (acidic) and Type II (basic) subfamilies, which pair to form heteropolymers essential for maintaining cellular structural integrity, mechanical stability, and stress resistance. CK-39 is predominantly expressed in stratified and glandular epithelia, such as those found in the prostate, bladder, and respiratory tract. Its expression pattern may vary depending on tissue differentiation states.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 39 antibody 86277-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |