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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOXC10 Recombinant antibody (86219-3-RR)

    WB analysis using 86219-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86219-3-RR (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HOXC10 Recombinant antibody (86219-3-RR)

    WB analysis using 86219-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86219-3-RR (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HOXC10 Recombinant antibody (86219-3-RR)

    IF Staining of HepG2 using 86219-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOXC10 antibody (86219-3-RR, Clone: 250873D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86219-3-RR

    Affinity and Kinetic Characterization of 86219-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86219-3-RR against Human HOXC10 were performed. The affinity constant is below 1 pM.

HOXC10 Recombinant monoclonal antibody

HOXC10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86219-3-RR
Clone No.250873D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Homeobox protein Hox-3I, Homeobox protein Hox-C10, HOX3I

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inT-47D cells, U2OS cells, HeLa cells， A375 cells, C2C12 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86219-3-RR targets HOXC10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2655

Product name: Recombinant human HOXC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-342 aa of BC001293

Sequence: MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTGNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKTINLTDRQVKIWFQNRRMKLKKMNRENRIRELTSNFNFT

相同性解析による交差性が予測される生物種
Full Name homeobox C10
Calculated molecular weight 342 aa, 38 kDa
Observed molecular weight50 kDa
GenBank accession numberBC001293
Gene Symbol HOXC10
Gene ID (NCBI) 3226
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NYD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486).

Protocols

Product Specific Protocols
IF protocol for HOXC10 antibody 86219-3-RRDownload protocol
WB protocol for HOXC10 antibody 86219-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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