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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CBFA2T3 Recombinant antibody (86198-4-RR)

    WB analysis using 86198-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86198-4-RR (CBFA2T3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CBFA2T3 Recombinant antibody (86198-4-RR)

    IF Staining of Jurkat using 86198-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CBFA2T3 antibody (86198-4-RR, Clone: 250859C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CBFA2T3 Recombinant monoclonal antibody

CBFA2T3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86198-4-RR
Clone No.250859C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

CBFA2T 3, ETO2, hMTG16, MTG16, MTG8 related protein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMOLT-4 cells, Jurkat cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86198-4-RR targets CBFA2T3 in applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9265

Product name: Recombinant human CBFA2T3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 267-615 aa of BC062624

Sequence: KRRTPDRTKENGSDRDPLHPQRYSPSNGPPQPTPPPHYRLEDIAMAHHFRDAYRHPDPRELRERHRPLVVPGSRQEEVIDHKLTEREWAEEWKHLNNLLNCIMDMVEKTRRSLTVLRRCQEADREELNHWARRYSDAEDTKKGPAPAAARPRSSSAGPEGPQLDVPREFLPRTLTGYVPEDIWRKAEEAVNEVKRQAMSELQKAVSDAERKAHELITTERAKMERALAEAKRQASEDALTVINQQEDSSESCWNCGRKASETCSGCNAARYCGSFCQHRDWEKHHHVCGQSLQGPTAVVADPVPGPPEAAHSLGPSLPVGAASPSEAGSAGPSRPGSPSPPGPLDTVPR

相同性解析による交差性が予測される生物種
Full Name core-binding factor, runt domain, alpha subunit 2; translocated to, 3
Calculated molecular weight 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC062624
Gene Symbol CBFA2T3
Gene ID (NCBI) 863
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO75081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CBFA2T3 antibody 86198-4-RRDownload protocol
WB protocol for CBFA2T3 antibody 86198-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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