Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, HepG2 cells, K-562 cells, NIH/3T3 cells, rat brain tissue, U-87 MG cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86196-1-RR targets SCPX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5895 Product name: Recombinant human SCPX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-322 aa of BC067108 Sequence: MSSSPWEPATLRRVFVVGVGMTKFVKPGAENSRDYPDLAEEAGKKALADAQIPYSAVDQACVGYVFGVAECVLALGFEKMSKGSLGIKFSDRTIPTDKHVDLLINKYGLSAHPVAPQMFGYAGKEHMEKYGTKIEHFAKIGWKNHKHSVNNPYSQFQDEYSLDEVMASKEVFDFLTILQCCPTSDGAAAAILASEAFVQKYGLQSKAVEILAQEMMTDLPSSFEEKSIIKMVGFDMSKEAARKCYEKSGLTPNDIDVIELHDCFSTNELLTYEALGLCPEGQGATLVDRGDNTYGGKWVINPSGGLISKGHPLGATGGHSCS 相同性解析による交差性が予測される生物種 |
| Full Name | sterol carrier protein 2 |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 46 kDa, 58 kDa |
| GenBank accession number | BC067108 |
| Gene Symbol | SCP2 |
| Gene ID (NCBI) | 6342 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22307 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCPX protein is a kind of protein related to cholesterol absorption and transport, belonging to SCP-2 gene family. SCPX protein contains an N-terminal thiolase domain and a C-terminal SCP-2 domain. In cells, SCPX protein will be cut into 44-46 kDa thiolase and 13-15 kDa SCP-2 protein. SCPX-2 is a nonspecific lipid transporter, which is involved in the transport and metabolism of intracellular lipids. It is distributed in peroxisome, cytoplasm and nucleus, and may play a role as a transcription factor in nucleus.