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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD93 Recombinant monoclonal antibody (86071-4-RR)

    WB analysis using 86071-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86071-4-RR (CD93 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using CD93 Recombinant monoclonal antibody (86071-4-RR)

    WB analysis of rat heart using 86071-4-RR

    rat heart tissue were subjected to SDS PAGE followed by western blot with 86071-4-RR (CD93 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CD93 Recombinant monoclonal antibody (86071-4-RR)

    WB analysis of mouse lung using 86071-4-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 86071-4-RR (CD93 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD93 Recombinant monoclonal antibody (86071-4-RR)

    IHC staining of mouse spleen using 86071-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86071-4-RR (CD93 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 86071-4-RR

    Affinity of 86071-4-RR

    Biolayer interferometry (BLl) kinetic assays of 86071-4-RR against Mouse CD93 were performed. The affinity constant is below 1 pM.

CD93 Recombinant monoclonal antibody

CD93 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 86071-4-RR
Clone No.250526C6

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Aa4, C1q/MBL/SPA receptor, C1qR(p), C1qr1, C1qRp

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat lung tissue, mouse lung tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86071-4-RR targets CD93 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3116

Product name: Recombinant Mouse CD93 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 23-572 aa of NM_010740.3

Sequence: ADSQAVVCEGTACYTAHWGKLSAAEAQHRCNENGGNLATVKSEEEARHVQQALTQLLKTKAPLEAKMGKFWIGLQREKGNCTYHDLPMRGFSWVGGGEDTAYSNWYKASKSSCIFKRCVSLILDLSLTPHPSHLPKWHESPCGTPEAPGNSIEGFLCKFNFKGMCRPLALGGPGRVTYTTPFQATTSSLEAVPFASVANVACGDEAKSETHYFLCNEKTPGIFHWGSSGPLCVSPKFGCSFNNGGCQQDCFEGGDGSFRCGCRPGFRLLDDLVTCASRNPCSSNPCTGGGMCHSVPLSENYTCRCPSGYQLDSSQVHCVDIDECQDSPCAQDCVNTLGSFHCECWVGYQPSGPKEEACEDVDECAAANSPCAQGCINTDGSFYCSCKEGYIVSGEDSTQCEDIDECSDARGNPCDSLCFNTDGSFRCGCPPGWELAPNGVFCSRGTVFSELPARPPQKEDNDDRKESTMPPTEMPSSPSGSKDVSNRAQTTGLFVQSDIPTASVPLEIEIPSEVSDVWFELGTYLPTTSGHSKPTHEDSVSAHSDTDGQN

相同性解析による交差性が予測される生物種
Full Name CD93 antigen
Calculated molecular weight69 kDa
Observed molecular weight90-120 kDa
GenBank accession numberNM_010740.3
Gene Symbol Cd93
Gene ID (NCBI) 17064
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO89103
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD93, also known as C1qR or C1qR(p), is a single-pass type I transmembrane glycoprotein and a member of the group 14 of the C-type lectin-like domain (CTLD) superfamily, which also includes endosialin/CD248, thrombomodulin, and CLEC14A (PMID: 31287944). CD93 consists of a C-type lectin-like domain, five EGF-like repeats, a mucin-like domain, a transmembrane domain and a cytoplasmic domain (PMID: 28912033). CD93 is mainly expressed on endothelial cells and has been reported to be expressed by neurons and various cells of the hematopoietic system, including monocytes, neutrophils, B cells, natural killer cells, platelets and hematopoietic stem cells (PMID: 37443812). CD93 plays a role in various physiological processes including angiogenesis, inflammation, and cell adhesion (PMID: 31287944).

Protocols

Product Specific Protocols
IHC protocol for CD93 antibody 86071-4-RRDownload protocol
WB protocol for CD93 antibody 86071-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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