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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Recombinant monoclonal antibody (86052-4-RR)

    IHC staining of human kidney using 86052-4-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Napsin A Recombinant monoclonal antibody (86052-4-RR)

    IHC staining of human kidney using 86052-4-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using Napsin A Recombinant monoclonal antibody (86052-4-RR)

    IHC staining of human lung using 86052-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using Napsin A Recombinant monoclonal antibody (86052-4-RR)

    IHC staining of human lung using 86052-4-RR

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 86052-4-RR (Napsin A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Napsin A Recombinant monoclonal antibody (86052-4-RR)

    IF Staining of human kidney using 86052-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Napsin A Recombinant monoclonal antibody (86052-4-RR)

    IF Staining of human kidney using 86052-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Napsin A Recombinant monoclonal antibody (86052-4-RR)

    IF Staining of A549 using 86052-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Napsin A antibody (86052-4-RR, Clone: 242433C10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 86052-4-RR

    Affinity of 86052-4-RR

    Biolayer interferometry (BLI) kinetic assays of 86052-4-RR against Human Napsin A were performed. The affinity constant is 0.149 nM.

Napsin A Recombinant monoclonal antibody

Napsin A Uni-rAb® Recombinant Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 86052-4-RR
Clone No.242433C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IF-P, ELISA

Synonyms

NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.-

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman lung tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86052-4-RR targets Napsin A in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9721

Product name: Recombinant human NAPSA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-420 aa of BC017842

Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG

相同性解析による交差性が予測される生物種
Full Name napsin A aspartic peptidase
Calculated molecular weight 420 aa, 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC017842
Gene Symbol Napsin A
Gene ID (NCBI) 9476
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO96009
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

Protocols

Product Specific Protocols
IF protocol for Napsin A antibody 86052-4-RRDownload protocol
IHC protocol for Napsin A antibody 86052-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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