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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1B1 Recombinant antibody (85916-1-RR)

    WB analysis using 85916-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP1B1 Recombinant antibody (85916-1-RR)

    WB analysis using 85916-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85916-1-RR (Atp1b1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ATP1B1 Recombinant antibody (85916-1-RR)

    IHC staining of mouse liver using 85916-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATP1B1 Recombinant antibody (85916-1-RR)

    IHC staining of mouse brain using 85916-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85916-1-RR (ATP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATP1B1 Recombinant monoclonal antibody

ATP1B1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85916-1-RR
Clone No.243093E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ATP1B 1, Atp4b, Beta 1 subunit of Na(+) K(+) ATPase, Na, K-ATPase β1, Na+/K+ ATPase beta 1 subunit

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, mouse heart tissue, rat heart tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85916-1-RR targets ATP1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3007

Product name: Recombinant Mouse Atp1b1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 63-304 aa of NM_009721.6

Sequence: ELKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIIRFLEKYKDSAQKDDMIFEDCGNVPSEPKERGDINHERGERKVCRFKLDWLGNCSGLNDDSYGYREGKPCIIIKLNRVLGFKPKPPKNESLETYPLMMKYNPNVLPVQCTGKRDEDKDKVGNIEYFGMGGYYGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTVDTEIRVECKAYGENIGYSEKDRFQGRFDVKIEIKS

相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, beta 1 polypeptide
Calculated molecular weight35 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberNM_009721.6
Gene Symbol Atp1b1
Gene ID (NCBI) 11931
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP14094
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

Protocols

Product Specific Protocols
WB protocol for ATP1B1 antibody 85916-1-RRDownload protocol
IHC protocol for ATP1B1 antibody 85916-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols