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Validation Data Gallery

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  • Sandwich ELISA sample test of MP02137-1

    Sandwich ELISA sample test of MP02137-1

    RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD74, and measured 14.17 ng/mL.

  • Sandwich ELISA sample test of MP02137-1

    Sandwich ELISA sample test of MP02137-1

    The mean CD74 concentration was determined to be 856.01 ng/mL in mouse thymus tissue extract based on a 5.80 mg/mL extract load and 789.39 ng/mL in mouse spleen tissue extract based on a 8.50 mg/mL extract load.

  • Sandwich ELISA sample test of MP02137-1

    Sandwich ELISA sample test of MP02137-1

    Serum of sixteen mice was measured. The CD74 concentration of detected samples was determined to be 21.51 pg/mL with a range of 12.61-42.94 pg/mL.

  • Sandwich ELISA standard curve of MP02137-1

    Sandwich ELISA standard curve of MP02137-1

    Sandwich ELISA standard curve of MP02137-1, Mouse CD74 Recombinant Matched Antibody Pair - PBS only. 85802-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2716. 85802-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD74 Recombinant monoclonal antibody (85802-2-RR)

    IHC staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD74 Recombinant monoclonal antibody (85802-2-RR)

    IHC staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85802-2-RR (CD74 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD74 Recombinant monoclonal antibody (85802-2-RR)

    IF Staining of mouse spleen using 85802-2-RR (same clone as 85802-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD74 antibody (85802-2-RR, Clone: 250109B11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85802-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02137-1

    Cytometric bead array standard curve of MP02137-1

    Cytometric bead array standard curve of MP02137-1, MOUSE CD74 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85802-1-PBS. Detection antibody: 85802-2-PBS. Standard: Eg2716. Range: 0.781-50 ng/mL.

Mouse CD74 Recombinant monoclonal antibody, PBS Only (Detector)

CD74 Uni-rAb® Recombinant Antibody for IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 85802-2-PBS
Clone No.250109B11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85802-2-PBS targets CD74 as part of a matched antibody pair:

MP02137-1: 85802-1-PBS capture and 85802-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2716

Product name: Recombinant Mouse Cd74 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 56-279 aa of NM_001042605.1

Sequence: QQQGRLDKLTITSQNLQLESLRMKLPKSAKPVSQMRMATPLLMRPMSMDNMLLGPVKNVTKYGNMTQDHVMHLLTRSGPLEYPQLKGTFPENLKHLKNSMDGVNWKIFESWMKQWLLFEMSKNSLEEKKPTEAPPKVLTKCQEEVSHIPAVYPGAFRPKCDENGNYLPLQCHGSTGYCWCVFPNGTEVPHTKSRGRHNCSEPLDMEDLSSGLGVTRQELGQVTL

相同性解析による交差性が予測される生物種
Full Name CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Calculated molecular weight32 kDa
GenBank accession numberNM_001042605.1
Gene Symbol Cd74
Gene ID (NCBI) 16149
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04441-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165; PMID: 36712241).

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