Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, C2C12 cells, PC-12 cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85686-1-RR targets DDX6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6211 Product name: Recombinant human P54 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 134-483 aa of BC065007 Sequence: RDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP 相同性解析による交差性が予測される生物種 |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 54 kDa |
| GenBank accession number | BC065007 |
| Gene Symbol | DDX6 |
| Gene ID (NCBI) | 1656 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26196 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDX6, also named as P54, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DDX6 antibody 85686-1-RR | Download protocol |
| WB protocol for DDX6 antibody 85686-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |