Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, SGC-7901 cells, K-562 cells, Sp2/0 cells |
| Positive IF/ICC detected in | A431 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85664-4-RR targets SII-TFIIS,TCEA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag12199 Product name: Recombinant human SII-TFIIS,TCEA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC072460 Sequence: MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC 相同性解析による交差性が予測される生物種 |
| Full Name | transcription elongation factor A (SII), 1 |
| Calculated molecular weight | 301 aa, 34 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC072460 |
| Gene Symbol | TCEA1 |
| Gene ID (NCBI) | 6917 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23193 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SII-TFIIS,TCEA1 antibody 85664-4-RR | Download protocol |
| WB protocol for SII-TFIIS,TCEA1 antibody 85664-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |