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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using COL4A1 Recombinant monoclonal antibody (85256-4-RR)

    WB analysis of HeLa using 85256-4-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85256-4-RR (COL4A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using COL4A1 Recombinant monoclonal antibody (85256-4-RR)

    IHC staining of human colon using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using COL4A1 Recombinant monoclonal antibody (85256-4-RR)

    IHC staining of human kidney using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using COL4A1 Recombinant monoclonal antibody (85256-4-RR)

    IHC staining of human placenta using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using COL4A1 Recombinant monoclonal antibody (85256-4-RR)

    IHC staining of human placenta using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85256-4-RR

    Affinity and Kinetic Characterization of 85256-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85256-4-RR against Human COL4A1 were performed. The affinity constant is 1.16 nM.

COL4A1 Recombinant monoclonal antibody

COL4A1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85256-4-RR
Clone No.251500E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

arresten, Collagen alpha 1(IV) chain, Collagen alpha-1(IV) chain, Collagen Type IV

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman placenta tissue, human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85256-4-RR targets COL4A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34391

Product name: Recombinant human COL4A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 901-1110 aa of BC151220

Sequence: LPGEKGDHGFPGSSGPRGDPGLKGDKGDVGLPGKPGSMDKVDMGSMKGQKGDQGEKGQIGPIGEKGSRGDPGTPGVPGKDGQAGQPGQPGPKGDPGISGTPGAPGLPGPKGSVGGMGLPGTPGEKGVPGIPGPQGSPGLPGDKGAKGEKGQAGPPGIGIPGLRGEKGDQGIAGFPGSPGEKGEKGSIGIPGMPGSPGLKGSPGSVGYPGS

相同性解析による交差性が予測される生物種
Full Name collagen, type IV, alpha 1
Calculated molecular weight 1669 aa, 161 kDa
Observed molecular weight220 kDa
GenBank accession numberBC151220
Gene Symbol COL4A1
Gene ID (NCBI) 1282
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02462
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL4A1 (Collagen Type IV Alpha 1 Chain) encodes a major component of type IV collagen, which is an essential structural element of basement membranes in various tissues. COL4A1 forms heterotrimers with other collagen type IV chains (e.g., COL4A2) and interacts with extracellular matrix components such as laminins, proteoglycans, and perlecans. Proteolytic cleavage of the non-collagenous domain of COL4A1 produces a fragment called arresten, which has anti-angiogenic and tumor suppressor properties. Elevated expression of COL4A1 has been observed in various cancers, including hepatocellular carcinoma (HCC), where it promotes tumor growth and metastasis through the FAK-Src signaling pathway. COL4A1 is highly expressed in various tissues, including the placenta, fat, and other organs. The expression of COL4A1 can be regulated by transcription factors such as RUNX1, which has been shown to upregulate COL4A1 in hepatocellular carcinoma.

Protocols

Product Specific Protocols
IHC protocol for COL4A1 antibody 85256-4-RRDownload protocol
WB protocol for COL4A1 antibody 85256-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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