Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, mouse uterus tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85203-1-RR targets CPSF7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | pre-mRNA cleavage factor I, 59 kDa subunit |
| Calculated molecular weight | 56 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | NM_001136040 |
| Gene Symbol | CPSF7 |
| Gene ID (NCBI) | 79869 |
| RRID | AB_3744100 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8N684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPSF7 antibody 85203-1-RR | Download protocol |
| WB protocol for CPSF7 antibody 85203-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |